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Potential Implications for Designing Drugs Against the Brown Spider Venom Phospholipase-D
(2017-04-01)
Loxoscelism refers to the clinical symptoms that develop after brown spider bites. Brown spider venoms contain several phospholipase-D isoforms, which are the main toxins responsible for both the cutaneous and systemic ...
A protective vaccine against the toxic activities following Brown spider accidents based on recombinant mutated phospholipases D as antigens
(2021-12-01)
Accidents involving Brown spiders are reported throughout the world. In the venom, the major toxins involved in the deleterious effects are phospholipases D (PLDs). In this work, recombinant mutated phospholipases D from ...
Group III PLA2 from the scorpion, Mesobuthus tamulus: cloning and recombinant expression in E. coli
(Pontificia Universidad Católica de Valparaíso, 2009)
Improved production of the recombinant phospholipase A1 from Polybia paulista wasp venom expressed in bacterial cells for use in routine diagnostics
(2020-05-01)
Phospholipase A1 (PLA1) is one of the three major allergens identified in the venom of P. paulista (Hymenoptera: Vespidae), a clinically relevant wasp from southeastern Brazil. The recombinant form of this allergen (rPoly ...
Molecular cloning, expression and IgE-immunoreactivity of phospholipase A1, a major allergen from Polybia paulista (Hymenoptera: Vespidae) venom
(2016-12-15)
Polybia paulista (Hymenoptera: Vespidae) is a clinically relevant social wasp that frequently causes stinging accidents in southeast Brazil. To date, diagnosis and specific immunotherapy (SIT) of allergy are based on the ...
Heterologous expression and biochemical and functional characterization of a recombinant alpha-type myotoxin inhibitor from Bothrops alternatus snake
(Elsevier B.V., 2014-10-01)
Venomous and non-venomous snakes possess phospholipase A(2) (PLA(2)) inhibitory proteins (PLIs) in their blood serum. This study shows the expression and biochemical and functional characterization of a recombinant alpha ...
Pichia pastoris engineering for the production of a modified phospholipase C
(Elsevier, 2016-12)
Crude vegetable oils are refined to remove impurities that adversely impact in their stability, color and flavor. In recent years, enzymatic degumming methods using phospholipase C (PLC) enzymes provide an environmentally ...
Brown spiders’ phospholipases-d with potential therapeutic applications: Functional assessment of mutant isoforms
(2021-03-01)
Phospholipases-D (PLDs) found in Loxosceles spiders’ venoms are responsible for the der-monecrosis triggered by envenomation. PLDs can also induce other local and systemic effects, such as massive inflammatory response, ...
Biological and structural comparison of recombinant phospholipase D toxins from Loxosceles intermedia (brown spider) venom
(Elsevier B.V., 2007-12-15)
The clinical features of brown spider bites are the appearance of necrotic skin lesions, which can also be accompanied by systemic involvement, including weakness, vomiting, fever, convulsions, disseminated intravascular ...